R-Loops as Cellular Regulators and Genomic Threats.
Mol Cell
; 73(3): 398-411, 2019 02 07.
Article
in En
| MEDLINE
| ID: mdl-30735654
During transcription, the nascent RNA strand can base pair with its template DNA, displacing the non-template strand as ssDNA and forming a structure called an R-loop. R-loops are common across many domains of life and cause DNA damage in certain contexts. In this review, we summarize recent results implicating R-loops as important regulators of cellular processes such as transcription termination, gene regulation, and DNA repair. We also highlight recent work suggesting that R-loops can be problematic to cells as blocks to efficient transcription and replication that trigger the DNA damage response. Finally, we discuss how R-loops may contribute to cancer, neurodegeneration, and inflammatory diseases and compare the available next-generation sequencing-based approaches to map R-loops genome wide.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
RNA
/
Cell Nucleus
/
Genome
/
Genomic Instability
/
Nucleic Acid Heteroduplexes
Limits:
Animals
/
Humans
Language:
En
Journal:
Mol Cell
Year:
2019
Document type:
Article