PBP Isolation and DD-Carboxypeptidase Assay.

Pal, Shilpa; Ghosh, Anindya S

Pal, Shilpa; Ghosh, Anindya S.

Affiliation

Pal S; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
Ghosh AS; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India. asghosh@bt.iitkgp.ac.in.

Methods Mol Biol ; 1946: 207-225, 2019.

Article in En | MEDLINE | ID: mdl-30798558

Subject(s)

Biological Assay; Carboxypeptidases/chemistry; Penicillin-Binding Proteins/chemistry; Penicillin-Binding Proteins/isolation & purification; Acylation; Biological Assay/methods; Cell Membrane/metabolism; Chromatography, Affinity; Cloning, Molecular; Gene Expression; Hydrolysis; Kinesis; Penicillin-Binding Proteins/genetics; Solubility

Key words

Acylation; Ampicillin-affinity chromatography; BOCILLIN; DD-Carboxypeptidase assay; Deacylation; Enzyme kinetics; Ni-NTA chromatography; Penicillin-binding protein (PBP)

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Assay / Carboxypeptidases / Penicillin-Binding Proteins Language: En Journal: Methods Mol Biol Year: 2019 Document type: Article

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