Nppb Neurons Are Sensors of Mast Cell-Induced Itch.
Cell Rep
; 26(13): 3561-3573.e4, 2019 03 26.
Article
in En
| MEDLINE
| ID: mdl-30917312
ABSTRACT
Itch is an unpleasant skin sensation that can be triggered by exposure to many chemicals, including those released by mast cells. The natriuretic polypeptide b (Nppb)-expressing class of sensory neurons, when activated, elicits scratching responses in mice, but it is unclear which itch-inducing agents stimulate these cells and the receptors involved. Here, we identify receptors expressed by Nppb neurons and demonstrate the functional importance of these receptors as sensors of endogenous pruritogens released by mast cells. Our search for receptors in Nppb neurons reveals that they express leukotriene, serotonin, and sphingosine-1-phosphate receptors. Targeted cell ablation, calcium imaging of primary sensory neurons, and conditional receptor knockout studies demonstrate that these receptors induce itch by the direct stimulation of Nppb neurons and neurotransmission through the canonical gastrin-releasing peptide (GRP)-dependent spinal cord itch pathway. Together, our results define a molecular and cellular pathway for mast cell-induced itch.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pruritus
/
Sensory Receptor Cells
/
Receptors, Atrial Natriuretic Factor
/
Receptors, Cell Surface
/
Mast Cells
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Cell Rep
Year:
2019
Document type:
Article