Vancomycin functionalized WO3 thin film-based impedance sensor for efficient capture and highly selective detection of Gram-positive bacteria.
Biosens Bioelectron
; 136: 23-30, 2019 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-31029006
ABSTRACT
In this study, we report a facile, reusable, and highly sensitive label-free impedance sensor for discriminating Gram-positive and Gram-negative bacteria. The impedance sensor was fabricated using gold interdigitated electrodes onto a tungsten oxide thin film. X-Ray diffraction confirmed the formation of polycrystalline tungsten oxide. Field emission scanning electron microscopy and atomic force microscopy revealed that tungsten oxide has a porous structure. Tungsten oxide was functionalized with vancomycin, a glycopeptide antibiotic known to have a specific interaction with the peptidoglycan layer of Gram-positive bacteria. Fourier transform infrared microscopy and scanning electron microscopy were employed to test the morphological coating of vancomycin on interdigitated electrodes/tungsten oxide sensor. The functionalized tungsten oxide sensor was highly efficient in the capture of Gram-positive bacteria. The impedance measurement was also sensitive to differentiate between viable and non-viable Gram-positive bacteria. Limit of detection 102 colony forming unit/ml, linear dynamic range 102-107 colony forming unit/ml under physiological conditions and reusable nature of this vancomycin coated impedance sensor provide a label-free strategy for quick, sensitive and highly selective detection of Gram-positive bacteria.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vancomycin
/
Gram-Positive Bacteria
/
Anti-Bacterial Agents
Type of study:
Diagnostic_studies
/
Evaluation_studies
Language:
En
Journal:
Biosens Bioelectron
Year:
2019
Document type:
Article