Specific detection of RNA mutation at single-base resolution by coupling the isothermal exponential amplification reaction (EXPAR) with chimeric DNA probe-aided precise RNA disconnection at the mutation site.

Chang, Fu; Sun, Yueying; Yang, Dandan; Yang, Wenjuan; Sun, Yuanyuan; Liu, Chenghui; Li, Zhengping

Chang, Fu; Sun, Yueying; Yang, Dandan; Yang, Wenjuan; Sun, Yuanyuan; Liu, Chenghui; Li, Zhengping.

Affiliation

Chang F; Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.

Chem Commun (Camb) ; 55(48): 6934-6937, 2019 Jun 11.

Article in En | MEDLINE | ID: mdl-31140481

ABSTRACT

ABSTRACT

A chimeric DNA probe-aided RNase H-EXPAR approach is developed for the accurate and specific detection of RNA mutation at single-base resolution through a new site-specific RNase H cleavage mechanism.

Subject(s)

DNA Probes/chemistry; Nucleotides/analysis; RNA/genetics; Base Sequence; DNA/chemistry; Fluorescent Dyes/chemistry; Mutation; Nucleic Acid Amplification Techniques/methods; RNA/chemistry; Ribonucleases/chemistry; Sensitivity and Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA / DNA Probes / Nucleotides Type of study: Diagnostic_studies Language: En Journal: Chem Commun (Camb) Year: 2019 Document type: Article

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