In Silico Prediction and In Vitro Assessment of Multifunctional Properties of Postbiotics Obtained From Two Probiotic Bacteria.
Probiotics Antimicrob Proteins
; 12(2): 608-622, 2020 06.
Article
in En
| MEDLINE
| ID: mdl-31280464
ABSTRACT
In this study, a global metabolite profile using Raman spectroscopy analysis was obtained in order to predict, by an in silico prediction of activity spectra for substance approach, the bioactivities of the intracellular content (IC) and cell wall (CW) fractions obtained from Lactobacillus casei CRL 431 and Bacillus coagulans GBI-30 strains. Additionally, multifunctional in vitro bioactivity of IC and CW fractions was also assessed. The metabolite profile revealed a variety of compounds (fatty acids, amino acids, coenzyme, protein, amino sugars), with significant probable activities (Pa > 0.7) as immune-stimulant, anti-inflammatory, neuroprotective, antiproliferative, immunomodulator, and antineoplastic, among others. Moreover, in vitro assays exhibited that both IC and CW fractions presented angiotensin-converting enzyme-inhibitory (> 90%), chelating (> 79%), and antioxidant (ca. 22-57 cellular antioxidant activity units) activities. Our findings based on in silico and in vitro analyses suggest that L. casei CRL 431 and B. coagulans GBI-30 strains appear to be promising sources of postbiotics and may impart health benefits by their multifunctional properties.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Probiotics
/
Metabolome
/
Bacillus coagulans
/
Lacticaseibacillus casei
Type of study:
Prognostic_studies
/
Risk_factors_studies
Language:
En
Journal:
Probiotics Antimicrob Proteins
Year:
2020
Document type:
Article