Targeted Metabolomics with Quantitative Dissolution Dynamic Nuclear Polarization.
Methods Mol Biol
; 2037: 385-393, 2019.
Article
in En
| MEDLINE
| ID: mdl-31463856
ABSTRACT
Metabolite profiles and their isotopomer distributions can be studied noninvasively in complex mixtures with NMR. The advent of hyperpolarized 13C-NMR using quantitative dissolution Dynamic Nuclear Polarization (qdDNP) and isotope enrichment add sensitivity to such metabolic studies, enabling mapping and quantification of metabolic pathways and networks. Here we describe a sample preparation method, including cell incubation, extraction, and signal enhancement, for reproducible and quantitative analysis of hyperpolarized 13C-NMR metabolite spectra. We further illustrate how qdDNP can be applied to gain metabolic insights into living cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carbon Isotopes
/
Magnetic Resonance Spectroscopy
/
Metabolic Networks and Pathways
/
Metabolomics
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Year:
2019
Document type:
Article