Propidium monoazide conventional PCR and DNA sequencing: detection of negative culture bacterial pathogens causing subclinical mastitis.

Farhan, M G; Abd El-Hamid, M I; Hassan, M N

Farhan, M G; Abd El-Hamid, M I; Hassan, M N.

Affiliation

Farhan MG; Department of Animal Management and Treatment, Fakous Veterinary Administration, Sharkia Governorate, Fakous, Egypt.
Abd El-Hamid MI; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Hassan MN; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.

J Appl Microbiol ; 128(6): 1595-1605, 2020 Jun.

Article in En | MEDLINE | ID: mdl-31965693

Subject(s)

Bacteria/isolation & purification; Bacteriological Techniques/veterinary; Food Microbiology/methods; Mastitis, Bovine/diagnosis; Milk/microbiology; Animals; Azides; Bacteria/classification; Bacteria/genetics; Bacteriological Techniques/methods; Cattle; Female; Mastitis, Bovine/microbiology; Polymerase Chain Reaction/methods; Polymerase Chain Reaction/veterinary; Propidium/analogs & derivatives; Sequence Analysis, DNA/veterinary

Key words

16S rRNA gene sequencing; PMA-PCR assay; mastitis; negative culture pathogens

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteria / Bacteriological Techniques / Milk / Food Microbiology / Mastitis, Bovine Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Journal: J Appl Microbiol Year: 2020 Document type: Article

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