Assessment of prevalence and load of torquetenovirus viraemia in a large cohort of healthy blood donors.
Clin Microbiol Infect
; 26(10): 1406-1410, 2020 Oct.
Article
in En
| MEDLINE
| ID: mdl-31972321
ABSTRACT
OBJECTIVES:
Torquetenovirus (TTV) is an emerging marker of functional immune competence with the potential to predict transplant-related adverse events. A large-scale epidemiological study was performed to understand how basal values vary in healthy individuals according to age and gender.METHODS:
We tested plasma from 1017 healthy blood donors aged 18-69 years. The presence and load of TTV were determined by a real-time PCR assay. A sub-cohort of 384 donors was tested for anti-cytomegalovirus IgG antibodies, and 100 participants were also tested for TTV viraemia on a paired whole blood sample.RESULTS:
The overall prevalence of TTV was 65% (657/1017) with a mean (±SD) growth of 5 ± 4% every 10 years of age increase, but stably higher in males (465/690, 67%) than in females (192/327, 59%). Mean (±SD) TTV load was 2.3 ± 0.7 Log copies/mL with no sex difference. TTV viraemia showed modest increases along 10-year age intervals (mean ± SD 0.3 ± 0.1). TTV viraemia in donors sampled 2 years later remained stable (mean ± SD 2.3 ± 0.8 versus 2.2 ± 0.7 Log copies between samples). Twenty-six per cent (9/34) of blood donors with TTV-negative plasma scored positive when whole blood was tested, and the donors with positive plasma showed a mean (±SD) 1.4 ± 0.5 Log increase in copy numbers when whole blood was tested.CONCLUSIONS:
This study establishes the mean value of TTV viraemia in plasma in healthy blood donors and suggests that ageing causes only minimal increases in TTV viraemia.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viremia
/
Blood Donors
/
DNA, Viral
/
Torque teno virus
/
DNA Virus Infections
Type of study:
Prevalence_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Clin Microbiol Infect
Year:
2020
Document type:
Article