An endoxylanase rapidly hydrolyzes xylan into major product xylobiose via transglycosylation of xylose to xylotriose or xylotetraose.

Zheng, Fengzhen; Song, Lina; Basit, Abdul; Liu, Junquan; Miao, Ting; Wen, Jiaqi; Cao, Yunhe; Jiang, Wei

Zheng, Fengzhen; Song, Lina; Basit, Abdul; Liu, Junquan; Miao, Ting; Wen, Jiaqi; Cao, Yunhe; Jiang, Wei.

Affiliation

Zheng F; Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: 18811068358@163.com.
Song L; Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: nnsong77@163.com.
Basit A; Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: abdul_9090@yahoo.com.
Liu J; Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: ljq0802040421@163.com.
Miao T; Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: 15501017617@163.com.
Wen J; Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: 15801029792@163.com.
Cao Y; State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China. Electronic address: caoyh@cau.edu.cn.
Jiang W; Beijing Advanced Innovation Center for Food Nutrition and Human Health, State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China. Electronic address: jiangwei01@cau.edu.cn.

Carbohydr Polym ; 237: 116121, 2020 Jun 01.

Article in En | MEDLINE | ID: mdl-32241400

Subject(s)

Disaccharides/biosynthesis; Endo-1,4-beta Xylanases/metabolism; Xylan Endo-1,3-beta-Xylosidase/metabolism; Xylans/metabolism; Cloning, Molecular; Hydrolysis; Kinetics; Pichia/genetics; Substrate Specificity; Trichoderma/enzymology; Trisaccharides/biosynthesis; Xylose/metabolism

Key words

Action mode; Biomass conversion; Catalysis mechanism; Improved expression; Sodium carboxymethyl cellulose (PubChem CID: 23706213); Xylanolytic enzyme; Xylobiose; Xylobiose (PubChem CID: 126963631); Xylopentaose (PubChem CID: 10146542); Xylose (PubChem CID: 644160); Xylotetraose (PubChem CID: 101601989); Xylotriose (PubChem CID: 12444993); pNP-α-l-arabinofuranoside (PubChem CID: 151437); pNP-ß-d- cellobioside (PubChem CID: 165125); pNP-ß-d-glucopyranoside (PubChem CID: 92930); pNP-ß-d-xylopyranoside (PubChem CID: 91509)

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Xylans / Endo-1,4-beta Xylanases / Xylan Endo-1,3-beta-Xylosidase / Disaccharides Language: En Journal: Carbohydr Polym Year: 2020 Document type: Article

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