Production of KRIT1-knockout and KRIT1-knockin Mouse Embryonic Fibroblasts as Cellular Models of CCM Disease.

Goitre, Luca; Fornelli, Claudia; Zotta, Alessia; Perrelli, Andrea; Retta, Saverio Francesco

Goitre, Luca; Fornelli, Claudia; Zotta, Alessia; Perrelli, Andrea; Retta, Saverio Francesco.

Affiliation

Goitre L; Department of Clinical and Biological Science, University of Torino, Orbassano (Torino), Italy. luca.goitre@gmail.com.
Fornelli C; CCM Italia Research Network, Torino, Italy. luca.goitre@gmail.com.
Zotta A; Department of Clinical and Biological Science, University of Torino, Orbassano (Torino), Italy.
Perrelli A; CCM Italia Research Network, Torino, Italy.
Retta SF; Department of Clinical and Biological Science, University of Torino, Orbassano (Torino), Italy.

Methods Mol Biol ; 2152: 151-167, 2020.

Article in En | MEDLINE | ID: mdl-32524551

Subject(s)

Fibroblasts/metabolism; Hemangioma, Cavernous, Central Nervous System/genetics; Hemangioma, Cavernous, Central Nervous System/metabolism; KRIT1 Protein/genetics; Animals; Disease Models, Animal; Gene Order; Gene Targeting; Genetic Loci; Genetic Vectors/genetics; Hemangioma, Cavernous, Central Nervous System/diagnosis; Homologous Recombination; Homozygote; Mice; Mice, Knockout; Mice, Transgenic; Transduction, Genetic

Key words

CCM genes; Cellular and animal models of CCM disease; Cerebral cavernous malformation (CCM); Cerebrovascular diseases; Heterozygous and homozygous KRIT1 knockout mice; KRIT1; KRIT1 knockout mouse embryonic fibroblast (MEF) cells

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Hemangioma, Cavernous, Central Nervous System / Fibroblasts / KRIT1 Protein Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Journal: Methods Mol Biol Year: 2020 Document type: Article

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