Transfer functions linking neural calcium to single voxel functional ultrasound signal.
Nat Commun
; 11(1): 2954, 2020 06 11.
Article
in En
| MEDLINE
| ID: mdl-32528069
Functional ultrasound imaging (fUS) is an emerging technique that detects changes of cerebral blood volume triggered by brain activation. Here, we investigate the extent to which fUS faithfully reports local neuronal activation by combining fUS and two-photon microscopy (2PM) in a co-registered single voxel brain volume. Using a machine-learning approach, we compute and validate transfer functions between dendritic calcium signals of specific neurons and vascular signals measured at both microscopic (2PM) and mesoscopic (fUS) levels. We find that transfer functions are robust across a wide range of stimulation paradigms and animals, and reveal a second vascular component of neurovascular coupling upon very strong stimulation. We propose that transfer functions can be considered as reliable quantitative reporters to follow neurovascular coupling dynamics.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Calcium
/
Ebolavirus
/
Neurons
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Nat Commun
Year:
2020
Document type:
Article