Analyzing the Impact of Protein Overexpression on Ca<sup>2+</sup> Dynamics and Development in Tobacco Pollen Tubes.

Zhang, Chunxia; Steinhorst, Leonie; Kudla, Jörg

Analyzing the Impact of Protein Overexpression on Ca²⁺ Dynamics and Development in Tobacco Pollen Tubes.

Zhang, Chunxia; Steinhorst, Leonie; Kudla, Jörg.

Affiliation

Zhang C; Institute of Plant Biology and Biotechnology, University of Münster, Münster, Germany.
Steinhorst L; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Kudla J; Institute of Plant Biology and Biotechnology, University of Münster, Münster, Germany.

Methods Mol Biol ; 2160: 223-231, 2020.

Article in En | MEDLINE | ID: mdl-32529440

Subject(s)

Calcium Signaling; Fluorescence Resonance Energy Transfer/methods; Plant Proteins/metabolism; Pollen Tube/metabolism; Up-Regulation; Fluorescence Resonance Energy Transfer/standards; Plant Proteins/genetics; Pollen Tube/genetics; Pollen Tube/physiology; Nicotiana

Key words

Ca2+ dynamics; Ca2+ indicator YC3.6; FRET Ca2+ imaging; Gold particle bombardment; Tobacco pollen tube

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Up-Regulation / Calcium Signaling / Fluorescence Resonance Energy Transfer / Pollen Tube Language: En Journal: Methods Mol Biol Year: 2020 Document type: Article

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