Molecular evidence for vaccine-induced canine distemper virus and canine adenovirus 2 coinfection in a fennec fox.
J Vet Diagn Invest
; 32(4): 598-603, 2020 Jul.
Article
in En
| MEDLINE
| ID: mdl-32560597
ABSTRACT
A 61-d-old fennec fox (Vulpes zerda), 11 d after receiving a multivalent, modified-live virus vaccine containing canine distemper virus (CDV), canine adenovirus 2 (CAdV-2), parainfluenza virus, parvovirus, and canine coronavirus, developed oculonasal discharge, and subsequently convulsions, and hemoptysis, and died. Microscopic changes in the cerebrum were evident, including neuronal degeneration and necrosis; intracytoplasmic eosinophilic inclusion bodies were observed in astrocytes. CDV was detected in the brain tissue by immunohistochemistry. Pulmonary lesions of multifocal necrotizing bronchopneumonia had Cowdry type A intranuclear inclusions in the bronchial epithelial cells. Electron microscopy revealed crystalline arrays of adenovirus-like particles within the intranuclear inclusions. Additionally, the hemagglutinin gene of CDV and the CAdV-2 DNA polymerase gene were detected in the fennec fox; sequence analysis showed 100% identity with those of the vaccine strain viruses. To our knowledge, vaccine-induced CDV and CAdV-2 coinfections using molecular analysis have not been reported previously. Therefore, vaccine strains should be considered prior to CDV vaccination in nondomestic carnivores.
Key words
Full text:
1
Collection:
01-internacional
Health context:
2_ODS3
/
4_TD
Database:
MEDLINE
Main subject:
Adenoviridae Infections
/
Adenoviruses, Canine
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Distemper Virus, Canine
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Coinfection
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Foxes
Limits:
Animals
Language:
En
Journal:
J Vet Diagn Invest
Year:
2020
Document type:
Article