tBHP treatment as a model for cellular senescence and pollution-induced skin aging.
Mech Ageing Dev
; 190: 111318, 2020 09.
Article
in En
| MEDLINE
| ID: mdl-32710895
ABSTRACT
Accumulation of senescent cells promotes the development of age-related pathologies and deterioration. In human skin, senescent cells potentially impair structure and function by secreting a mixture of signaling molecules and proteases that influence neighboring cells and degrade extracellular matrix components, such as elastin and collagen. One of the key underlying mechanisms of senescence and extrinsic skin aging is the increase of intracellular reactive oxygen species and resulting oxidative stress. Tert-butyl hydroperoxide (tBHP) is a known inducer of oxidative stress and cellular damage, acting at least in part by depleting the antioxidant glutathione. Here, we provide a detailed characterization of tBHP-induced senescence in human dermal fibroblasts in monolayer culture. In addition, results obtained with more physiological experimental models revealed that tBHP treated 3D reconstructed skin and ex vivo skin developed signs of chronic tissue damage, displaying reduced epidermal thickness and collagen fiber thinning. We, therefore, propose that tBHP treatment can be used as a model to study the effects of extrinsic skin aging, focusing mainly on the influence of environmental pollution.
Key words
Full text:
1
Collection:
01-internacional
Health context:
2_ODS3
Database:
MEDLINE
Main subject:
Skin
/
Skin Aging
/
Tert-Butylhydroperoxide
/
Environmental Pollution
/
Fibroblasts
/
Glutathione
Aspects:
Determinantes_sociais_saude
Limits:
Humans
Language:
En
Journal:
Mech Ageing Dev
Year:
2020
Document type:
Article