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Evaluation of regenerated tracheal cilia function on a collagen-conjugated scaffold in a canine model.
Ueda, Yuichiro; Sato, Toshihiko; Nakamura, Ryosuke; Tamari, Shigeyuki; Muranishi, Yusuke; Yutaka, Yojiro; Nakamura, Tatsuo; Omori, Koichi; Iwasaki, Akinori; Date, Hiroshi.
Affiliation
  • Ueda Y; Department of Thoracic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.
  • Sato T; Department of General Thoracic Surgery, Breast and Pediatric Surgery, Fukuoka University School of Medicine, Fukuoka, Japan.
  • Nakamura R; Department of Organ and Tissue Reconstruction, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.
  • Tamari S; Department of General Thoracic Surgery, Breast and Pediatric Surgery, Fukuoka University School of Medicine, Fukuoka, Japan.
  • Muranishi Y; Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
  • Yutaka Y; Department of Thoracic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.
  • Nakamura T; Department of Thoracic Surgery, Kyoto City Hospital, Kyoto, Japan.
  • Omori K; Department of Thoracic Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan.
  • Iwasaki A; Department of Organ and Tissue Reconstruction, Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan.
  • Date H; Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Interact Cardiovasc Thorac Surg ; 31(5): 644-649, 2020 11 01.
Article in En | MEDLINE | ID: mdl-32888291
OBJECTIVES: It is unclear whether the movement and function of the regenerated cilia on collagen-conjugated artificial trachea are the same as those of normal cilia. This study assessed the ciliary beat frequency (CBF) and ciliary transport functions (CTFs) of regenerated cilia in a canine model. METHODS: A tracheal defect introduced into the anterior portion of the cervical trachea of an adult beagle dog was covered with a collagen-conjugated prosthesis. Two months later, the trachea was harvested along the long axis, both from normal and regenerated regions. The cilia were stained with isothiocyanate-conjugated wheat germ agglutinin, and their movement was monitored with a high-speed camera to analyse CBF and CTF. Four samples each were obtained from the regenerated and normal regions for CBF analysis and 7 samples each were obtained for CTF analysis. RESULTS: The wheat germ agglutinin-stained cells showed well-regulated beats in both the regenerated and normal regions of the trachea. Mean CBF in the regenerated and normal regions did not differ significantly (7.11 ± 0.41 vs 7.14 ± 1.09 Hz; P = 981). By contrast, CTF was significantly lower in the regenerated region than in the normal region (30.0 ± 6.62 vs 7.43 ± 0.58 µm/s; P = 0.005). CONCLUSIONS: Mean CBF in the regenerated and normal regions did not differ significantly at 2 months. The CTF in the regenerated region recovered partially but remained lower than those in the normal region. Methods are needed to improve the CTF of regenerated cilia.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trachea / Collagen / Cilia / Guided Tissue Regeneration / Tissue Scaffolds Limits: Animals Language: En Journal: Interact Cardiovasc Thorac Surg Year: 2020 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trachea / Collagen / Cilia / Guided Tissue Regeneration / Tissue Scaffolds Limits: Animals Language: En Journal: Interact Cardiovasc Thorac Surg Year: 2020 Document type: Article