Your browser doesn't support javascript.
loading
Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti.
Koh, Cassandra; Islam, M Nurul; Ye, Yixin H; Chotiwan, Nunya; Graham, Barbara; Belisle, John T; Kouremenos, Konstantinos A; Dayalan, Saravanan; Tull, Dedreia L; Klatt, Stephan; Perera, Rushika; McGraw, Elizabeth A.
Affiliation
  • Koh C; School of Biological Sciences, Monash University, Clayton, VIC, 3800, Australia.
  • Islam MN; Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, 80523, USA.
  • Ye YH; School of Biological Sciences, Monash University, Clayton, VIC, 3800, Australia.
  • Chotiwan N; Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, 80523, USA.
  • Graham B; Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, 80523, USA.
  • Belisle JT; Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, 80523, USA.
  • Kouremenos KA; Metabolomics Australia, Bio21 Institute of Molecular Sciences and Biotechnology, University of Melbourne, Parkville, VIC, 3010, Australia.
  • Dayalan S; Metabolomics Australia, Bio21 Institute of Molecular Sciences and Biotechnology, University of Melbourne, Parkville, VIC, 3010, Australia.
  • Tull DL; Metabolomics Australia, Bio21 Institute of Molecular Sciences and Biotechnology, University of Melbourne, Parkville, VIC, 3010, Australia.
  • Klatt S; Department of Biochemistry and Molecular Biology, Bio21 Institute of Molecular Sciences and Biotechnology, University of Melbourne, Parkville, VIC, 3010, Australia.
  • Perera R; The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, 3052, Australia.
  • McGraw EA; Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, 80523, USA. rushika.perera@colostate.edu.
Commun Biol ; 3(1): 518, 2020 09 18.
Article in En | MEDLINE | ID: mdl-32948809
Competition between viruses and Wolbachia for host lipids is a proposed mechanism of Wolbachia-mediated virus blocking in insects. Yet, the metabolomic interaction between virus and symbiont within the mosquito has not been clearly defined. We compare the lipid profiles of Aedes aegypti mosquitoes bearing mono- or dual-infections of the Wolbachia wMel strain and dengue virus serotype 3 (DENV3). We found metabolic signatures of infection-induced intracellular events but little evidence to support direct competition between Wolbachia and virus for host lipids. Lipid profiles of dual-infected mosquitoes resemble those of DENV3 mono-infected mosquitoes, suggesting virus-driven modulation dominates over that of Wolbachia. Interestingly, knockdown of key metabolic enzymes suggests cardiolipins are host factors for DENV3 and Wolbachia replication. These findings define the Wolbachia-DENV3 metabolic interaction as indirectly antagonistic, rather than directly competitive, and reveal new research avenues with respect to mosquito × virus interactions at the molecular level.
Subject(s)
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Health context: 2_ODS3 / 3_ND / 4_TD Database: MEDLINE Main subject: Aedes / Wolbachia / Dengue Virus / Lipid Metabolism Limits: Animals / Humans Language: En Journal: Commun Biol Year: 2020 Document type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Health context: 2_ODS3 / 3_ND / 4_TD Database: MEDLINE Main subject: Aedes / Wolbachia / Dengue Virus / Lipid Metabolism Limits: Animals / Humans Language: En Journal: Commun Biol Year: 2020 Document type: Article