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Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma.
Saito, Yoshimasa; Muramatsu, Toshihide; Saito, Hidetsugu.
Affiliation
  • Saito Y; Division of Pharmacotherapeutics, Keio University Faculty of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
  • Muramatsu T; Division of Pharmacotherapeutics, Keio University Faculty of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
  • Saito H; Division of Pharmacotherapeutics, Keio University Faculty of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.
STAR Protoc ; 1(1): 100009, 2020 06 19.
Article in En | MEDLINE | ID: mdl-33111070
ABSTRACT
This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biliary Tract / Biliary Tract Neoplasms / Carcinoma / Organoids / Cell Culture Techniques / Tissue Culture Techniques Limits: Humans Language: En Journal: STAR Protoc Year: 2020 Document type: Article
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biliary Tract / Biliary Tract Neoplasms / Carcinoma / Organoids / Cell Culture Techniques / Tissue Culture Techniques Limits: Humans Language: En Journal: STAR Protoc Year: 2020 Document type: Article