Identification of regulators of poly-ADP-ribose polymerase inhibitor response through complementary CRISPR knockout and activation screens.

Clements, Kristen E; Schleicher, Emily M; Thakar, Tanay; Hale, Anastasia; Dhoonmoon, Ashna; Tolman, Nathanial J; Sharma, Anchal; Liang, Xinwen; Imamura Kawasawa, Yuka; Nicolae, Claudia M; Wang, Hong-Gang; De, Subhajyoti; Moldovan, George-Lucian

Clements, Kristen E; Schleicher, Emily M; Thakar, Tanay; Hale, Anastasia; Dhoonmoon, Ashna; Tolman, Nathanial J; Sharma, Anchal; Liang, Xinwen; Imamura Kawasawa, Yuka; Nicolae, Claudia M; Wang, Hong-Gang; De, Subhajyoti; Moldovan, George-Lucian.

Affiliation

Clements KE; Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Schleicher EM; Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Thakar T; Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Hale A; Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Dhoonmoon A; Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Tolman NJ; Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Sharma A; Rutgers Cancer Institute of New Jersey, Rutgers the State University of New Jersey, New Brunswick, NJ, 08901, USA.
Liang X; Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Imamura Kawasawa Y; Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Nicolae CM; Department of Pharmacology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Wang HG; Institute for Personalized Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
De S; Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Moldovan GL; Department of Pediatrics, The Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.

Nat Commun ; 11(1): 6118, 2020 11 30.

Article in En | MEDLINE | ID: mdl-33257658

Subject(s)

Clustered Regularly Interspaced Short Palindromic Repeats; Poly(ADP-ribose) Polymerase Inhibitors/isolation & purification; Poly(ADP-ribose) Polymerase Inhibitors/pharmacology; Poly(ADP-ribose) Polymerases/drug effects; BRCA2 Protein/genetics; BRCA2 Protein/metabolism; Biomarkers; DNA Damage; DNA Repair; Gene Knockout Techniques; HeLa Cells; Homologous Recombination/drug effects; Humans; Lysine Acetyltransferase 5/metabolism; Mad2 Proteins/metabolism; Rad51 Recombinase/genetics; Rad51 Recombinase/metabolism; Tumor Suppressor Proteins/genetics; Tumor Suppressor Proteins/metabolism; Tumor Suppressor p53-Binding Protein 1; Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Poly(ADP-ribose) Polymerases / Clustered Regularly Interspaced Short Palindromic Repeats / Poly(ADP-ribose) Polymerase Inhibitors Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Nat Commun Year: 2020 Document type: Article

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