An optimized workflow for CRISPR-Cas9 deletion of surface and intracellular factors in primary human T lymphocytes.

Leoni, Cristina; Bianchi, Niccolò; Vincenzetti, Lucia; Monticelli, Silvia

Leoni, Cristina; Bianchi, Niccolò; Vincenzetti, Lucia; Monticelli, Silvia.

Affiliation

Leoni C; Institute for Research in Biomedicine (IRB), Università della Svizzera italiana (USI), Bellinzona, Switzerland.
Bianchi N; Institute for Research in Biomedicine (IRB), Università della Svizzera italiana (USI), Bellinzona, Switzerland.
Vincenzetti L; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
Monticelli S; Institute for Research in Biomedicine (IRB), Università della Svizzera italiana (USI), Bellinzona, Switzerland.

PLoS One ; 16(2): e0247232, 2021.

Article in En | MEDLINE | ID: mdl-33600503

Subject(s)

CRISPR-Cas Systems/genetics; Gene Editing/methods; Membrane Proteins/genetics; Transcription Factors/genetics; Cell Cycle Proteins/deficiency; Cell Cycle Proteins/genetics; Cells, Cultured; Endoribonucleases/deficiency; Endoribonucleases/genetics; Humans; Membrane Proteins/deficiency; RNA, Guide, Kinetoplastida/genetics; RNA, Guide, Kinetoplastida/metabolism; Receptors, Antigen, T-Cell, alpha-beta/deficiency; Receptors, Antigen, T-Cell, alpha-beta/genetics; T-Box Domain Proteins/deficiency; T-Box Domain Proteins/genetics; T-Lymphocytes/cytology; T-Lymphocytes/metabolism; Transcription Factors/deficiency

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / CRISPR-Cas Systems / Gene Editing / Membrane Proteins Type of study: Prognostic_studies Limits: Humans Language: En Journal: PLoS One Year: 2021 Document type: Article

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