Your browser doesn't support javascript.
loading
Different Influences of trans Fatty Acids on the Phospholipase A2 and Arachidonic Acid Metabolic Pathway in Hepatocytes.
Zou, Qian; Wei, Meng; Zhang, Niu; Niu, Xian; Weng, Chen; Deng, Ze-Yuan; Li, Jing.
Affiliation
  • Zou Q; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
  • Wei M; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
  • Zhang N; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
  • Niu X; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
  • Weng C; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
  • Deng ZY; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
  • Li J; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
J Agric Food Chem ; 69(14): 4120-4133, 2021 Apr 14.
Article in En | MEDLINE | ID: mdl-33819034
ABSTRACT
This study investigated the effects of 9t181 (representing I-TFAs), 9t161, and 11t181 (representing R-TFAs) and their mixtures on the normal human hepatocyte LO2 cell function, the possible mechanism of lipid metabolism by lipidomics, and the relationship between phospholipase A2 (PLA2) and the arachidonic acid (AA) metabolic pathway. Here, we found that the damaging effect of 9t181 on the LO2 cell function was significantly greater than those of 11t181 and 9t161 (p < 0.05), and the damaging effects of CHB and HSO were significantly greater than those of HHB and CM (p < 0.05). The lipidomic results showed that TFAs and TFA mixtures caused a significant change in the lipid profiles of LO2 cells, in which the TAG, PL, and OL contents increased significantly. Moreover, 9t181 regulated only the protein expression of cPLA2 but did not participate in the AA metabolic pathway, while 11t181 and 9t161 participated in the COX-2 and CYP450 pathways, respectively.
Subject(s)
Key words
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trans Fatty Acids Limits: Humans Language: En Journal: J Agric Food Chem Year: 2021 Document type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trans Fatty Acids Limits: Humans Language: En Journal: J Agric Food Chem Year: 2021 Document type: Article