Carbamoylated chitosan hydrogels with improved viscoelastic properties and stability for potential 3D cell culture applications.
Biomed Mater
; 16(4)2021 05 07.
Article
in En
| MEDLINE
| ID: mdl-33857925
ABSTRACT
We demonstrate a benign and straightforward method to modify the chitosan (CH) by carbamoylation. The free amines on CH are converted into carbamyl functionalities by reacting with potassium cyanate (KCNO). One wt% CH solution, when reacted with KCNO ⩾ 0.1 M, leads to the sol-gel transition of CH through the hydrogen bonding to form carbamoylated chitosan (CCH) hydrogel. Gelation time of CCH decreases with an increase in the KCNO concentration and an interconnected porous network is formed as observed under SEM. Rheological studies show that while one wt% CH solution is a viscous liquid, the CCH hydrogel with 0.5 M KCNO has a storage modulus (G') of 104Pa. The CCH hydrogel is proved to be non-cytotoxic and promotes the attachment and growth of the small lung cancer model A549, and the neuroblastoma SH-SY5Y cell lines. CCH hydrogel also promotes the differentiation of SH-SY5Y cells into neuronal cells, as supported by immunostaining and thus demonstrating its utility as a versatile scaffold for three-dimensional cell-culture systems.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biocompatible Materials
/
Hydrogels
/
Chitosan
/
Cell Culture Techniques, Three Dimensional
Limits:
Humans
Language:
En
Journal:
Biomed Mater
Year:
2021
Document type:
Article