Identification of Extrachromosomal Circular Forms of Active Transposable Elements Using Mobilome-Seq.
Methods Mol Biol
; 2250: 87-93, 2021.
Article
in En
| MEDLINE
| ID: mdl-33900594
ABSTRACT
Active transposable elements (TEs) generate insertion polymorphisms that can be detected through genome resequencing strategies. However, these techniques may have limitations for organisms with large genomes or for somatic insertions. Here, we present a method that takes advantage of the extrachromosomal circular DNA (eccDNA) forms of actively transposing TEs in order to detect and characterize active TEs in any plant or animal tissue. Mobilome-seq consists in selectively amplifying and sequencing eccDNAs. It relies on linear digestion of genomic DNA followed by rolling circle amplification of circular DNA. Both active DNA transposons and retrotransposons can be identified using this technique.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Circular
/
DNA Transposable Elements
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Year:
2021
Document type:
Article