Methylobrevis albus sp. nov., isolated from freshwater lake sediment.

ABSTRACT

An aerobic, Gram-stain-negative, rod-shaped bacterium with flagellum, designated L22T, was isolated from sediment of Hulun Lake, Inner Mongolia, China. The organism was found to grow optimally at 30° C in a medium containing 0-0.75% (w/v) NaCl at pH 7.5. The major fatty acid identified was summed feature 8 (C181ω7c). The dominant polar lipids were phosphomonoester, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The main respiratory quinone was Q-10. The draft genome sequence of strain L22T consisted of 4354,788 bp. The G + C content of genomic DNA was 69.8 mol %. The 16S rRNA gene sequences indicated that strain L22T was affiliated with the genus Methylobrevis within the family Pleomorphomonadaceae, being most closely related to Methylobrevis pamukkalensis JCM 30229T with 95.9% 16S rRNA gene sequences similarity. The AAI, ANI and dDDH values between strain L22T and M. pamukkalensis JCM 30229T were 72.5%, 80.7% and 22.7%. Based on taxonomic results in this study, we proposed that strain L22T a novel species in the genus Methylobrevis of the family Pleomorphomonadaceae, for which the name Methylobrevis albus sp. nov. is proposed. The type strain is L22T (=KCTC 72858T=MCCC 1H00432T).