Bioinformatic Pipelines to Analyze lncRNAs RNAseq Data.
Methods Mol Biol
; 2348: 55-69, 2021.
Article
in En
| MEDLINE
| ID: mdl-34160799
ABSTRACT
RNA-sequencing could be nowadays considered the gold standard to study the coding and noncoding transcriptome. The great advantage of high-throughput sequencing in the characterization and quantification of long noncoding RNA (lncRNA) resides in its capability to capture the complexity of lncRNA transcripts configuration patterns, even in the presence of several alternative isoforms, with superior accuracy and discovery power compared to other technologies such as microarrays or PCR-based methods. In this chapter, we provide a protocol for lncRNA analysis using through high-throughput sequencing, indicating the main difficulties in the annotation pipeline and showing how an accurate evaluation of the procedure can help to minimize biased observations.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sequence Analysis, RNA
/
Computational Biology
/
Gene Expression Profiling
/
Transcriptome
/
RNA, Long Noncoding
Language:
En
Journal:
Methods Mol Biol
Year:
2021
Document type:
Article