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High-fat diet-induced colonocyte dysfunction escalates microbiota-derived trimethylamine N-oxide.
Yoo, Woongjae; Zieba, Jacob K; Foegeding, Nora J; Torres, Teresa P; Shelton, Catherine D; Shealy, Nicolas G; Byndloss, Austin J; Cevallos, Stephanie A; Gertz, Erik; Tiffany, Connor R; Thomas, Julia D; Litvak, Yael; Nguyen, Henry; Olsan, Erin E; Bennett, Brian J; Rathmell, Jeffrey C; Major, Amy S; Bäumler, Andreas J; Byndloss, Mariana X.
Affiliation
  • Yoo W; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Zieba JK; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Foegeding NJ; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Torres TP; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Shelton CD; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Shealy NG; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Byndloss AJ; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Cevallos SA; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Gertz E; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Tiffany CR; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Thomas JD; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Litvak Y; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Nguyen H; Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, CA 95616, USA.
  • Olsan EE; Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, CA 95616, USA.
  • Bennett BJ; Department of Biological Sciences, California State University, Sacramento, CA 95819, USA.
  • Rathmell JC; Agriculture Research Service (ARS-USDA), University of California at Davis, Davis, CA 95616, USA.
  • Major AS; Department of Nutrition, University of California at Davis, Davis, CA 95616, USA.
  • Bäumler AJ; Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, CA 95616, USA.
  • Byndloss MX; Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Science ; 373(6556): 813-818, 2021 08 13.
Article in En | MEDLINE | ID: mdl-34385401
A Western-style, high-fat diet promotes cardiovascular disease, in part because it is rich in choline, which is converted to trimethylamine (TMA) by the gut microbiota. However, whether diet-induced changes in intestinal physiology can alter the metabolic capacity of the microbiota remains unknown. Using a mouse model of diet-induced obesity, we show that chronic exposure to a high-fat diet escalates Escherichia coli choline catabolism by altering intestinal epithelial physiology. A high-fat diet impaired the bioenergetics of mitochondria in the colonic epithelium to increase the luminal bioavailability of oxygen and nitrate, thereby intensifying respiration-dependent choline catabolism of E. coli In turn, E. coli choline catabolism increased levels of circulating trimethlamine N-oxide, which is a potentially harmful metabolite generated by gut microbiota.
Subject(s)

Full text: 1 Collection: 01-internacional Health context: 3_ND Database: MEDLINE Main subject: Colon / Escherichia coli / Diet, High-Fat / Intestinal Mucosa / Methylamines Limits: Animals Language: En Journal: Science Year: 2021 Document type: Article

Full text: 1 Collection: 01-internacional Health context: 3_ND Database: MEDLINE Main subject: Colon / Escherichia coli / Diet, High-Fat / Intestinal Mucosa / Methylamines Limits: Animals Language: En Journal: Science Year: 2021 Document type: Article