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Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence.
Neumann, Elizabeth K; Patterson, Nathan Heath; Allen, Jamie L; Migas, Lukasz G; Yang, Haichun; Brewer, Maya; Anderson, David M; Harvey, Jennifer; Gutierrez, Danielle B; Harris, Raymond C; deCaestecker, Mark P; Fogo, Agnes B; Van de Plas, Raf; Caprioli, Richard M; Spraggins, Jeffrey M.
Affiliation
  • Neumann EK; Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
  • Patterson NH; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA.
  • Allen JL; Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
  • Migas LG; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA.
  • Yang H; Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
  • Brewer M; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA.
  • Anderson DM; Delft Center for Systems and Control (DCSC), Delft University of Technology, 2628 CD Delft, the Netherlands.
  • Harvey J; Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Gutierrez DB; Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
  • Harris RC; Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
  • deCaestecker MP; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA.
  • Fogo AB; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA.
  • Van de Plas R; Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.
  • Caprioli RM; Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA.
  • Spraggins JM; Division of Nephrology and Hypertension, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
STAR Protoc ; 2(3): 100747, 2021 09 17.
Article in En | MEDLINE | ID: mdl-34430920
ABSTRACT
Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ system. Through careful optimization, we ensure high-quality and reproducible datasets suitable for cross-patient comparisons that are essential to understanding human health and disease. Moreover, each of these steps can be adapted to other organ systems or diseases, enabling additional atlas efforts.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Specimen Handling / Fluorescent Antibody Technique / Multimodal Imaging / Kidney Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: STAR Protoc Year: 2021 Document type: Article
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Specimen Handling / Fluorescent Antibody Technique / Multimodal Imaging / Kidney Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: STAR Protoc Year: 2021 Document type: Article