Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence.
STAR Protoc
; 2(3): 100747, 2021 09 17.
Article
in En
| MEDLINE
| ID: mdl-34430920
ABSTRACT
Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ system. Through careful optimization, we ensure high-quality and reproducible datasets suitable for cross-patient comparisons that are essential to understanding human health and disease. Moreover, each of these steps can be adapted to other organ systems or diseases, enabling additional atlas efforts.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Specimen Handling
/
Fluorescent Antibody Technique
/
Multimodal Imaging
/
Kidney
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
STAR Protoc
Year:
2021
Document type:
Article