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Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection.
Zhong, Xiaotian; Schwab, Ashley; Ma, Weijun; Meade, Caryl L; Zhou, Jing; D'Antona, Aaron M; Somers, Will; Lin, Laura.
Affiliation
  • Zhong X; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA. xiaotian.zhong@pfizer.com.
  • Schwab A; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
  • Ma W; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
  • Meade CL; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
  • Zhou J; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
  • D'Antona AM; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
  • Somers W; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
  • Lin L; BioMedicine Design, Medicinal Sciences, Pfizer Worldwide R&D, Cambridge, MA, USA.
Methods Mol Biol ; 2313: 143-150, 2022.
Article in En | MEDLINE | ID: mdl-34478135
ABSTRACT
Large-scale transient expression in Chinese Hamster Ovary (CHO) cells provides a rapid protein production method with a potential start-to-end alignment advantage for biotherapeutics drug discovery. In this chapter, experimental protocols are illustrated for transient expression of therapeutic glycoproteins with improved galactosylation and sialylation in ExpiCHO-S™ system. To reduce the production cost, we also describe a novel procedure for PEI-mediated transfection in ExpiCHO-S™ cells that supports therapeutic protein expression comparable to the level with ExpiFectamine™-based transfection.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transfection Type of study: Guideline Limits: Animals Language: En Journal: Methods Mol Biol Year: 2022 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transfection Type of study: Guideline Limits: Animals Language: En Journal: Methods Mol Biol Year: 2022 Document type: Article