The identification of grain size genes by RapMap reveals directional selection during rice domestication.
Nat Commun
; 12(1): 5673, 2021 09 28.
Article
in En
| MEDLINE
| ID: mdl-34584089
ABSTRACT
Cloning quantitative trait locus (QTL) is time consuming and laborious, which hinders the understanding of natural variation and genetic diversity. Here, we introduce RapMap, a method for rapid multi-QTL mapping by employing F2 gradient populations (F2GPs) constructed by minor-phenotypic-difference accessions. The co-segregation standard of the single-locus genetic models ensures simultaneous integration of a three-in-one framework in RapMap i.e. detecting a real QTL, confirming its effect, and obtaining its near-isogenic line-like line (NIL-LL). We demonstrate the feasibility of RapMap by cloning eight rice grain-size genes using 15 F2GPs in three years. These genes explain a total of 75% of grain shape variation. Allele frequency analysis of these genes using a large germplasm collection reveals directional selection of the slender and long grains in indica rice domestication. In addition, major grain-size genes have been strongly selected during rice domestication. We think application of RapMap in crops will accelerate gene discovery and genomic breeding.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oryza
/
Selection, Genetic
/
Edible Grain
/
Computational Biology
/
Quantitative Trait Loci
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
Nat Commun
Year:
2021
Document type:
Article