Your browser doesn't support javascript.
loading
Molecular analysis of sugar transporters and glycolysis pathways in Ettlia sp. under heterotrophy using fructose and glucose.
Kim, Minsik; Jeon, Seungjib; Kang, Nam Kyu; Lee, Hyung-Gwan; Shin, Won-Sub; Koh, Hyun Gi; Yun, Jin-Ho; Ahn, Chi-Yong; Oh, Hee-Mock; Chang, Yong Keun.
Affiliation
  • Kim M; Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Daejeon, 34141, Republic of Korea.
  • Jeon S; Cell Factory Research Center, Korea Research Institute for Bioscience and Bioengineering (KRIBB), 125 Gwahak-ro, Daejeon, 34141, Republic of Korea.
  • Kang NK; Research Institute of Industrial Technology Convergence, Korea Institute of Industrial Technology (KITECH), 143 Hanggaul-ro, Ansan, Gyeonggi-do, 15588, Republic of Korea.
  • Lee HG; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
  • Shin WS; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
  • Koh HG; Cell Factory Research Center, Korea Research Institute for Bioscience and Bioengineering (KRIBB), 125 Gwahak-ro, Daejeon, 34141, Republic of Korea.
  • Yun JH; Advanced Biomass R&D Center, 291 Daehak-ro, Daejeon, 34141, Republic of Korea.
  • Ahn CY; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
  • Oh HM; DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
  • Chang YK; Cell Factory Research Center, Korea Research Institute for Bioscience and Bioengineering (KRIBB), 125 Gwahak-ro, Daejeon, 34141, Republic of Korea.
Biotechnol J ; 17(1): e2100214, 2022 Jan.
Article in En | MEDLINE | ID: mdl-34669258
ABSTRACT
Fructophilic behavior in microalgae is a rare trait that could benefit biorefineries by enabling substitution of carbon source with fructose, and our previous study identified that Ettlia sp. prefers fructose relative to glucose. In this study, by analyzing the transcription levels of genes related to sugar transport and the glycolysis pathway, the fructose utilization of Ettlia sp. was investigated. In a fructose-containing medium, the expression levels of fructokinase (EttFRK3) and glucokinase (EttGCK1 and EttGCK2) genes were significantly upregulated in heterotrophic cultivation of Ettlia sp. under fructose supplementation conditions. Further, a sugar transporter (EttSTF11) was significantly upregulated by 3.2-fold in 1 day, and this increase was analogous to the specific growth rate exhibited by the species. Subsequent cultivation tests with multi-sugar sources also showed a significant upregulation of EttSTF11 relative to other treatments without fructose. A phylogenetic tree derived from the analysis of different transporters of interest identified that EttSTF11 was adjacent to reference fructose transporters with a high bootstrap value of 71. Given that the transmembrane domains of EttSTF11 were analogous to those of reference fructose transporter genes, EttSTF11 appeared to play a critical role in fructose consumption and metabolism in Ettlia sp.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fructose / Glucose Language: En Journal: Biotechnol J Year: 2022 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fructose / Glucose Language: En Journal: Biotechnol J Year: 2022 Document type: Article