Your browser doesn't support javascript.
loading
Genetic architecture modulates diet-induced hepatic mRNA and miRNA expression profiles in Diversity Outbred mice.
Que, Excel; James, Kristen L; Coffey, Alisha R; Smallwood, Tangi L; Albright, Jody; Huda, M Nazmul; Pomp, Daniel; Sethupathy, Praveen; Bennett, Brian J.
Affiliation
  • Que E; Western Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Davis, CA 95616, USA.
  • James KL; Department of Nutrition, University of California, Davis, Davis, CA 95616, USA.
  • Coffey AR; Department of Nutrition, University of California, Davis, Davis, CA 95616, USA.
  • Smallwood TL; Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 28081, USA.
  • Albright J; Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 28081, USA.
  • Huda MN; Nutrition Research Institute, University of North Carolina at Chapel Hill, Kannapolis, NC 28081, USA.
  • Pomp D; Western Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Davis, CA 95616, USA.
  • Sethupathy P; Department of Nutrition, University of California, Davis, Davis, CA 95616, USA.
  • Bennett BJ; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Genetics ; 218(3)2021 07 14.
Article in En | MEDLINE | ID: mdl-34849860
Genetic approaches in model organisms have consistently demonstrated that molecular traits such as gene expression are under genetic regulation, similar to clinical traits. The resulting expression quantitative trait loci (eQTL) have revolutionized our understanding of genetic regulation and identified numerous candidate genes for clinically relevant traits. More recently, these analyses have been extended to other molecular traits such as protein abundance, metabolite levels, and miRNA expression. Here, we performed global hepatic eQTL and microRNA expression quantitative trait loci (mirQTL) analysis in a population of Diversity Outbred mice fed two different diets. We identified several key features of eQTL and mirQTL, namely differences in the mode of genetic regulation (cis or trans) between mRNA and miRNA. Approximately 50% of mirQTL are regulated by a trans-acting factor, compared to ∼25% of eQTL. We note differences in the heritability of mRNA and miRNA expression and variance explained by each eQTL or mirQTL. In general, cis-acting variants affecting mRNA or miRNA expression explain more phenotypic variance than trans-acting variants. Finally, we investigated the effect of diet on the genetic architecture of eQTL and mirQTL, highlighting the critical effects of environment on both eQTL and mirQTL. Overall, these data underscore the complex genetic regulation of two well-characterized RNA classes (mRNA and miRNA) that have critical roles in the regulation of clinical traits and disease susceptibility.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Multifactorial Inheritance / MicroRNAs / Diet / Liver Type of study: Prognostic_studies Limits: Animals Language: En Journal: Genetics Year: 2021 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Multifactorial Inheritance / MicroRNAs / Diet / Liver Type of study: Prognostic_studies Limits: Animals Language: En Journal: Genetics Year: 2021 Document type: Article