Anti-osteoclastogenic cycloartane saponins from Mussaenda pubescens.
Nat Prod Res
; 36(18): 4597-4604, 2022 Sep.
Article
in En
| MEDLINE
| ID: mdl-34898346
ABSTRACT
The methanolic extract of Mussaenda pubescens Dryand leaves exhibited significant anti-osteoclastogenic activity. Chemical investigation of M. pubescens led to the isolation of one new cycloartane saponin, mussaendoside X (1) along with eight known compounds heinsiagenin A 3-O-[α-L-rhamnopyranosyl-(1â2)-O-ß-D-glucopyranosyl-(1â2)]-O-ß-D-glucopyranoside (2), mussaendoside O (3), heinsiagenin A 3-O-[α-L-rhamnopyranosyl-(1â2)-O-ß-D-glucopyranosyl-(1â2)]-O-ß-D-glucopyranosyl-(1â4)-O-ß-D-glucopyranoside (4), mussaendoside G (5), mussaendoside U (6), shanzhiside methyl ester (7), barlerin (8) and musaenoside (9). Their structures were elucidated by extensive spectroscopic methods including 1D- and 2D-NMR as well as MS analysis and comparison with the literature. Cycloartane saponins 1-6 positively suppressed osteoclast formation in an anti-osteoclastogenic screening assay. Consequently, treatment of RANKL-stimulated RAW 264.7 cells with compounds 1-4 significantly decreased the number of osteoclasts in a concentration-dependent manner. Six compounds from M. pubescens, with the new cycloartane, mussaendoside X, were shown for the first time as potential effective inhibitors of osteoclastogenesis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saponins
/
Triterpenes
/
Rubiaceae
Language:
En
Journal:
Nat Prod Res
Year:
2022
Document type:
Article