Vacancy-Induced Visible Light-Driven Fluorescence in Toxic Ion-Free Resorbable Magnetic Calcium Phosphates for Cell Imaging Applications.
ACS Appl Bio Mater
; 4(4): 3256-3263, 2021 04 19.
Article
in En
| MEDLINE
| ID: mdl-35014412
ABSTRACT
Multifunctional nanosized particles are very beneficial in the field of biomedicine. Bioactive and highly biocompatible calcium phosphate (CaP) nanoparticles (â¼50 nm) exhibiting both superparamagnetic and fluorescence properties were synthesized by incorporating dual ions (Fe3+ and Sr2+) in HAp (hydroxyapatite) [Ca10(PO4)6(OH)2]. Insertion of Fe3+ creates oxygen vacancies at the PO43- site, thereby destabilizing the structure. Thus, in order to maintain the structural stability, Sr2+ has been incorporated. This incorporation of Sr2+ leads to an intense emission at 550 nm. HAp nanoparticles when subjected to thermal treatment (800 °C) transform to ß-TCP, exhibiting emission at 710 nm due to the emergence of an intermediate band. Moreover, these nanoparticles exhibit fluorescence in visible light when compared to the other UV and IR fluorescence excitation sources which could damage the tissues. The synthesis involving the combination of ultrasound and microwave techniques resulted in the distribution of Fe3+ in the interstitial sites of CaP, which is responsible for the excellent fluorescent properties. Moreover, thermally treated CaP becomes superparamagnetic, without affecting the desired optical properties. The bioactive, biocompatible, magnetic, and fluorescent properties of this resorbable CaP which is free from toxic heavy metals (Eu, Gd, etc.) could help in overcoming the long-term cytotoxicity. This could also be useful in tracking the location of the nanoparticles during drug delivery and magnetic hyperthermia. The bioactive fluorescent CaP nanoparticle helps in monitoring the bone growth and in addition, it could be employed in cell imaging applications. The in vitro MCF-7 imaging using the nanoparticles after 24 h of uptake at 465 nm evidences the bioimaging capability of the prepared nanoparticles. The reproducibility of the defect level is essential for the defect-induced emission properties. The synthesis of nontoxic fluorescent CaP is highly reproducible with the present synthesis method. Hence, it could be safely employed in various biomedical applications.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biocompatible Materials
/
Calcium Phosphates
/
Optical Imaging
/
Fluorescence
/
Light
Limits:
Humans
Language:
En
Journal:
ACS Appl Bio Mater
Year:
2021
Document type:
Article