Combined active pocket and hinge region engineering to develop an NADPH-dependent phenylglycine dehydrogenase.

Yin, Xinjian; Zeng, Yujing; Chen, Jun; Liu, Lan; Gao, Zhizeng

Yin, Xinjian; Zeng, Yujing; Chen, Jun; Liu, Lan; Gao, Zhizeng.

Affiliation

Yin X; School of Marine Science, Sun Yat-sen University, Zhuhai 519080, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519080, China.
Zeng Y; School of Marine Science, Sun Yat-sen University, Zhuhai 519080, China.
Chen J; School of Marine Science, Sun Yat-sen University, Zhuhai 519080, China.
Liu L; School of Marine Science, Sun Yat-sen University, Zhuhai 519080, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519080, China.
Gao Z; School of Marine Science, Sun Yat-sen University, Zhuhai 519080, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519080, China. Electronic address: gaozhizeng@mail.sysu.edu.cn.

Bioorg Chem ; 120: 105601, 2022 03.

Article in En | MEDLINE | ID: mdl-35033816

Subject(s)

Glutamate Dehydrogenase; NADPH Dehydrogenase; Amino Acid Oxidoreductases/chemistry; Amino Acid Oxidoreductases/metabolism; Amino Acids/metabolism; Glutamate Dehydrogenase/chemistry; Glutamate Dehydrogenase/metabolism; Kinetics; NADP/metabolism; NADPH Dehydrogenase/metabolism

Key words

Glutamate dehydrogenase; Hinge region; Noncanonical amino acids; Protein engineering; l-Phenylglycine

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Glutamate Dehydrogenase / NADPH Dehydrogenase Language: En Journal: Bioorg Chem Year: 2022 Document type: Article

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