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Direct adenylation from 5'-OH-terminated oligonucleotides by a fusion enzyme containing Pfu RNA ligase and T4 polynucleotide kinase.
Yang, Zhengquan; Zhang, Chengliang; Lian, Guojun; Dong, Shijie; Song, Menghui; Shao, Hengrong; Wang, Jingmei; Zhong, Tao; Luo, Zhenni; Jin, Shengnan; Ding, Chunming.
Affiliation
  • Yang Z; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Zhang C; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Lian G; Department of Clinical Laboratory, Kunming Third People's Hospital, Kunming, Yunnan, 650041, China.
  • Dong S; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Song M; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Shao H; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Wang J; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Zhong T; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Luo Z; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Jin S; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
  • Ding C; Key Laboratory of Laboratory Medicine, Ministry of Education of China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Nucleic Acids Res ; 50(13): 7560-7569, 2022 07 22.
Article in En | MEDLINE | ID: mdl-35819229
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligonucleotides / Polynucleotide 5'-Hydroxyl-Kinase / Adenosine Monophosphate / Genetic Techniques / MicroRNAs Language: En Journal: Nucleic Acids Res Year: 2022 Document type: Article
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligonucleotides / Polynucleotide 5'-Hydroxyl-Kinase / Adenosine Monophosphate / Genetic Techniques / MicroRNAs Language: En Journal: Nucleic Acids Res Year: 2022 Document type: Article