[CircRNA-0028171 regulates arsenic trioxide-induced apoptosis in vascular endothelial cells].
Sheng Li Xue Bao
; 74(5): 763-772, 2022 Oct 25.
Article
in Zh
| MEDLINE
| ID: mdl-36319099
ABSTRACT
The present study was aimed to investigate the effects of circRNA-0028171 on the apoptosis of vascular endothelial cells induced by arsenic trioxide (As2O3). Human umbilical vein endothelial cells (HUVECs) were treated with 0-15 µmol/L As2O3 for 24 h. Then, cellular viability was measured by MTT assay. The expression levels of circRNA-0028171, Bcl-2 and Bax mRNA were detected by real-time quantitative PCR. Bcl-2/Bax protein ratio was detected by Western blot. Whether circRNA-0028171 was involved in the regulation of HUVECs by As2O3 was investigated by transfection with overexpression plasmid of circRNA-0028171 and siRNA. The results showed that compared with the control group, As2O3 group showed decreased cellular viability, reduced Bcl-2/Bax mRNA and protein ratios, and significantly lower expression of circRNA-0028171. Overexpression of circRNA-0028171 inhibited apoptosis of HUVECs induced by As2O3. Knockdown of circRNA-0028171 by siRNA promoted As2O3-induced apoptosis in HUVECs. These results suggest that circRNA-0028171 is involved in the vascular endothelial cell apoptosis induced by As2O3.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apoptosis
/
RNA, Circular
Limits:
Humans
Language:
Zh
Journal:
Sheng Li Xue Bao
Year:
2022
Document type:
Article