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COVID-19 diagnostics: Molecular biology to nanomaterials.
Perveen, Summaya; Negi, Anjali; Gopalakrishnan, Varun; Panda, Satyajit; Sharma, Vinay; Sharma, Rashmi.
Affiliation
  • Perveen S; Infectious Diseases Division, CSIR- Indian Institute of Integrative Medicine, Jammu 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
  • Negi A; Infectious Diseases Division, CSIR- Indian Institute of Integrative Medicine, Jammu 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
  • Gopalakrishnan V; Department of Materials Science and Engineering, University of Illinois Urbana-Champaign, United States.
  • Panda S; Metallurgical and Materials Engineering, National Institute of Technology Rourkela, India.
  • Sharma V; Department of Biosciences and Bioengineering, Indian Institute of Technology Jammu, Jammu, India. Electronic address: vinay.sharma@iitjammu.ac.in.
  • Sharma R; Infectious Diseases Division, CSIR- Indian Institute of Integrative Medicine, Jammu 180001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India. Electronic address: rashmi.sharma.09@iiim.res.in.
Clin Chim Acta ; 538: 139-156, 2023 Jan 01.
Article in En | MEDLINE | ID: mdl-36403665
The SARS-CoV-2 pandemic has claimed around 6.4 million lives worldwide. The disease symptoms range from mild flu-like infection to life-threatening complications. The widespread infection demands rapid, simple, and accurate diagnosis. Currently used methods include molecular biology-based approaches that consist of conventional amplification by RT-PCR, isothermal amplification-based techniques such as RT-LAMP, and gene editing tools like CRISPR-Cas. Other methods include immunological detection including ELISA, lateral flow immunoassay, chemiluminescence, etc. Radiological-based approaches are also being used. Despite good analytical performance of these current methods, there is an unmet need for less costly and simpler tests that may be performed at point of care. Accordingly, nanomaterial-based testing has been extensively pursued. In this review, we discuss the currently used diagnostic techniques for SARS-CoV-2, their usefulness, and limitations. In addition, nanoparticle-based approaches have been highlighted as another potential means of detection. The review provides a deep insight into the current diagnostic methods and future trends to combat this deadly menace.
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Full text: 1 Collection: 01-internacional Health context: 4_TD Database: MEDLINE Main subject: Nanoparticles / COVID-19 Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Clin Chim Acta Year: 2023 Document type: Article

Full text: 1 Collection: 01-internacional Health context: 4_TD Database: MEDLINE Main subject: Nanoparticles / COVID-19 Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Clin Chim Acta Year: 2023 Document type: Article