In Situ Identification of Secondary Structures in Unpurified Bombyx mori Silk Fibrils Using Polarized Two-Dimensional Infrared Spectroscopy.
Biomacromolecules
; 23(12): 5340-5349, 2022 Dec 12.
Article
in En
| MEDLINE
| ID: mdl-36437734
ABSTRACT
The mechanical properties of biomaterials are dictated by the interactions and conformations of their building blocks, typically proteins. Although the macroscopic behavior of biomaterials is widely studied, our understanding of the underlying molecular properties is generally limited. Among the noninvasive and label-free methods to investigate molecular structures, infrared spectroscopy is one of the most commonly used tools because the absorption bands of amide groups strongly depend on protein secondary structure. However, spectral congestion usually complicates the analysis of the amide spectrum. Here, we apply polarized two-dimensional (2D) infrared spectroscopy (IR) to directly identify the protein secondary structures in native silk films cast from Bombyx mori silk feedstock. Without any additional peak fitting, we find that the initial effect of hydration is an increase of the random coil content at the expense of the helical content, while the ß-sheet content is unchanged and only increases at a later stage. This paper demonstrates that 2D-IR can be a valuable tool for characterizing biomaterials.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bombyx
/
Fibroins
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Biomacromolecules
Year:
2022
Document type:
Article