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Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia.
Wannigama, Dhammika Leshan; Amarasiri, Mohan; Hongsing, Parichart; Hurst, Cameron; Modchang, Charin; Chadsuthi, Sudarat; Anupong, Suparinthon; Phattharapornjaroen, Phatthranit; S M, Ali Hosseini Rad; Fernandez, Stefan; Huang, Angkana T; Kueakulpattana, Naris; Tanasatitchai, Chanikan; Vatanaprasan, Porames; Saethang, Thammakorn; Luk-In, Sirirat; Storer, Robin James; Ounjai, Puey; Ragupathi, Naveen Kumar Devanga; Kanthawee, Phitsanuruk; Sano, Daisuke; Furukawa, Takashi; Sei, Kazunari; Leelahavanichkul, Asada; Kanjanabuch, Talerngsak; Hirankarn, Nattiya; Higgins, Paul G; Kicic, Anthony; Chatsuwan, Tanittha; McLellan, Alexander D; Abe, Shuichi.
Affiliation
  • Wannigama DL; Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Center of Excellence in Antimic
  • Amarasiri M; Laboratory of Environmental Hygiene, Department of Health Science, School of Allied Health Sciences, Graduate School of Medical Sciences, Kitasato University, Kitasato, Sagamihara-Minami, Kanagawa 252-0373, Japan.
  • Hongsing P; Mae Fah Luang University Hospital, Chiang Rai, Thailand; School of Integrative Medicine, Mae Fah Luang University, Chiang Rai, Thailand.
  • Hurst C; Molly Wardaguga Research Centre, Charles Darwin University, Queensland, Australia; Statistics, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
  • Modchang C; Biophysics Group, Department of Physics, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Centre of Excellence in Mathematics, MHESI, Bangkok 10400, Thailand; Thailand Center of Excellence in Physics, Ministry of Higher Education, Science, Research and Innovation, 328 Si Ayutthaya Ro
  • Chadsuthi S; Department of Physics, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
  • Anupong S; Biophysics Group, Department of Physics, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
  • Phattharapornjaroen P; Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; Institute of Clinical Sciences, Department of Surgery, Sahlgrenska Academy, Gothenburg University, 40530 Gothenburg, Sweden.
  • S M AHR; Department of Microbiology and Immunology, University of Otago, Dunedin 9010, Otago, New Zealand; Center of Excellence in Immunology and Immune-Mediated Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
  • Fernandez S; Department of Virology, U.S. Army Medical Directorate, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Huang AT; Department of Virology, U.S. Army Medical Directorate, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
  • Kueakulpattana N; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Center of Excellence in Antimicrobial Resistance and Stewardship, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Tanasatitchai C; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Center of Excellence in Antimicrobial Resistance and Stewardship, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Vatanaprasan P; Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
  • Saethang T; Department of Computer Science, Faculty of Science, Kasetsart University, Bangkok, Thailand.
  • Luk-In S; Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
  • Storer RJ; Office of Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Ounjai P; Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.
  • Ragupathi NKD; School of Medicine, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield, United Kingdom; Department of Clinical Microbiology, Christian Medical C
  • Kanthawee P; School of Health Science, Mae Fah Luang University, Thailand.
  • Sano D; Department of Frontier Sciences for Advanced Environment, Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan; Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, Sendai, Miyagi, Japan.
  • Furukawa T; Laboratory of Environmental Hygiene, Department of Health Science, School of Allied Health Sciences, Graduate School of Medical Sciences, Kitasato University, Kitasato, Sagamihara-Minami, Kanagawa 252-0373, Japan.
  • Sei K; Laboratory of Environmental Hygiene, Department of Health Science, School of Allied Health Sciences, Graduate School of Medical Sciences, Kitasato University, Kitasato, Sagamihara-Minami, Kanagawa 252-0373, Japan.
  • Leelahavanichkul A; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Translational Research in Inflammation and Immunology Research Unit (TRIRU), Department of Microbiology, Chulalongkorn University, Bangkok, Thai
  • Kanjanabuch T; Division of Nephrology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Center of Excellence in Kidney Metabolic Disorders, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Dialysis Policy and Practice Program (DiP3), School of Global Health
  • Hirankarn N; Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan; Center of Excellence in Immunology and Immune-Mediated Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
  • Higgins PG; Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany; German Centre for Infection Research, Partner site Bonn-Cologne, Cologne, Germany.
  • Kicic A; Telethon Kids Institute, University of Western Australia, Nedlands, 6009, Western Australia, Australia; School of Population Health, Curtin University, Bentley 6102, Western Australia, Australia; Centre for Cell Therapy and Regenerative Medicine, Medical School, The University of Western Australia,
  • Chatsuwan T; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Center of Excellence in Antimicrobial Resistance and Stewardship, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Electronic a
  • McLellan AD; Department of Microbiology and Immunology, University of Otago, Dunedin 9010, Otago, New Zealand.
  • Abe S; Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan; Pathogen Hunter's Research Collaborative Team, Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan.
Sci Total Environ ; 858(Pt 1): 159816, 2023 Feb 01.
Article in En | MEDLINE | ID: mdl-36461562
ABSTRACT
The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mpox (monkeypox) Limits: Humans Country/Region as subject: Asia Language: En Journal: Sci Total Environ Year: 2023 Document type: Article
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mpox (monkeypox) Limits: Humans Country/Region as subject: Asia Language: En Journal: Sci Total Environ Year: 2023 Document type: Article