Coupled induction of prophage and virulence factors during tick transmission of the Lyme disease spirochete.
Nat Commun
; 14(1): 198, 2023 01 13.
Article
in En
| MEDLINE
| ID: mdl-36639656
ABSTRACT
The alternative sigma factor RpoS plays a central role in the critical host-adaptive response of the Lyme disease spirochete, Borrelia burgdorferi. We previously identified bbd18 as a negative regulator of RpoS but could not inactivate bbd18 in wild-type spirochetes. In the current study we employed an inducible bbd18 gene to demonstrate the essential nature of BBD18 for viability of wild-type spirochetes in vitro and at a unique point in vivo. Transcriptomic analyses of BBD18-depleted cells demonstrated global induction of RpoS-dependent genes prior to lysis, with the absolute requirement for BBD18, both in vitro and in vivo, circumvented by deletion of rpoS. The increased expression of plasmid prophage genes and the presence of phage particles in the supernatants of lysing cultures indicate that RpoS regulates phage lysis-lysogeny decisions. Through this work we identify a mechanistic link between endogenous prophages and the RpoS-dependent adaptive response of the Lyme disease spirochete.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ticks
/
Borrelia burgdorferi
/
Prophages
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Nat Commun
Year:
2023
Document type:
Article