5'-End Mapping in Human Mitochondrial DNA.
Methods Mol Biol
; 2615: 315-325, 2023.
Article
in En
| MEDLINE
| ID: mdl-36807801
ABSTRACT
Here, we describe an assay that enables mapping of 5'-ends across the genome using next-generation sequencing on an Illumina platform, 5'-End-sequencing (5'-End-seq). We use this method to map free 5'-ends in mtDNA isolated from fibroblasts. This method can be used to answer key questions regarding DNA integrity, DNA replication mechanisms and to identify priming events, primer processing, nick processing, and double strand break processing on the entire genome.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Mitochondrial
/
Mitochondria
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Year:
2023
Document type:
Article