Low genetic heterogeneity of Leishmania major in different geographical regions of Iran.
PLoS One
; 18(5): e0285520, 2023.
Article
in En
| MEDLINE
| ID: mdl-37155684
ABSTRACT
To examine the genetic diversity of Leishmania major, 100 Giemsa-stained positive slides were collected from endemic foci of Iran (Northeast, Central, and Southwest provinces) over two consecutive years during 2019-2021. The Leishmania ITS-rDNA gene was amplified and Leishmania sp. was recognized by PCR-RFLP and sequencing. In addition, 178 registered ITS-rDNA sequences from other geographical regions of Iran were retrieved from GenBank, including different host species (human, sandfly and rodent). A total of 40 new haplotypes were discovered using the ITS-rDNA sequence analysis. IR29 (20.6%) and IR34 (61%) were the two most common haplotypes, represented by a star-like feature in the overall population. Analysis of the molecular variance test revealed low genetic diversity of L. major in human cases (Haplotype diversity; 0.341), rodent (Hd; 0.387) and sandfly (Hd; 0.390) sequences. The lowest genetic diversity of L. major was observed in Southwest/Southeast Iran (Hd 0.104-0.286). The statistically Fst value indicated that L. major is not genetically differentiated between geographic regions of Iran, except for the Northeast-Southwest (Fst 0.29055) and Central-Southwest (Fst 0.30294) population pairs. The current study as the first investigation discloses new perspectives for further evaluation in the identification local transmission paradigms and initiating effective prevention strategies.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phlebotomus
/
Psychodidae
/
Leishmaniasis, Cutaneous
/
Leishmania major
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
PLoS One
Year:
2023
Document type:
Article