Skin Whole-Mount Immunofluorescent Staining Protocol, 3D Visualization, and Spatial Image Analysis.

Schmidt, Alfonso J; Wright, Graham D; Ronchese, Franca; Price, Kylie M

Schmidt, Alfonso J; Wright, Graham D; Ronchese, Franca; Price, Kylie M.

Affiliation

Schmidt AJ; Hugh Green Cytometry Centre, Wellington, New Zealand.
Wright GD; Malaghan Institute of Medical Research, Wellington, New Zealand.
Ronchese F; A*STAR Microscopy Platform, Research Support Centre, Agency for Science, Technology & Research (A*STAR), 30 Biopolis Street, #05-02D Matrix, 138671, Singapore.
Price KM; Malaghan Institute of Medical Research, Wellington, New Zealand.

Curr Protoc ; 3(6): e820, 2023 Jun.

Article in En | MEDLINE | ID: mdl-37338194

Subject(s)

Imaging, Three-Dimensional; Skin; Animals; Mice; Imaging, Three-Dimensional/methods; Skin/diagnostic imaging; Staining and Labeling; Coloring Agents; Microscopy, Confocal/methods

Key words

FIJI; Neighborhood Frequency and Normalized Median Evenness (NME); Spatial Distribution Index (SDI); cell profiler; confocal laser scanning microscopy (CLSM); fluorescence-conjugated primary antibody; image analysis; polychromatic immunofluorescent staining; whole-mount skin

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Skin / Imaging, Three-Dimensional Limits: Animals Language: En Journal: Curr Protoc Year: 2023 Document type: Article

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