Cell-type specific defects in PTEN-mutant cortical organoids converge on abnormal circuit activity.
Hum Mol Genet
; 32(18): 2773-2786, 2023 09 05.
Article
in En
| MEDLINE
| ID: mdl-37384417
ABSTRACT
De novo heterozygous loss-of-function mutations in phosphatase and tensin homolog (PTEN) are strongly associated with autism spectrum disorders; however, it is unclear how heterozygous mutations in this gene affect different cell types during human brain development and how these effects vary across individuals. Here, we used human cortical organoids from different donors to identify cell-type specific developmental events that are affected by heterozygous mutations in PTEN. We profiled individual organoids by single-cell RNA-seq, proteomics and spatial transcriptomics and revealed abnormalities in developmental timing in human outer radial glia progenitors and deep-layer cortical projection neurons, which varied with the donor genetic background. Calcium imaging in intact organoids showed that both accelerated and delayed neuronal development phenotypes resulted in similar abnormal activity of local circuits, irrespective of genetic background. The work reveals donor-dependent, cell-type specific developmental phenotypes of PTEN heterozygosity that later converge on disrupted neuronal activity.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autism Spectrum Disorder
/
Neurons
Limits:
Humans
Language:
En
Journal:
Hum Mol Genet
Year:
2023
Document type:
Article