Lactate-dependent transcriptional regulation controls mammalian eye morphogenesis.
Nat Commun
; 14(1): 4129, 2023 07 14.
Article
in En
| MEDLINE
| ID: mdl-37452018
ABSTRACT
Mammalian retinal metabolism favors aerobic glycolysis. However, the role of glycolytic metabolism in retinal morphogenesis remains unknown. We report that aerobic glycolysis is necessary for the early stages of retinal development. Taking advantage of an unbiased approach that combines the use of eye organoids and single-cell RNA sequencing, we identify specific glucose transporters and glycolytic genes in retinal progenitors. Next, we determine that the optic vesicle territory of mouse embryos displays elevated levels of glycolytic activity. At the functional level, we show that removal of Glucose transporter 1 and Lactate dehydrogenase A gene activity from developing retinal progenitors arrests eye morphogenesis. Surprisingly, we uncover that lactate-mediated upregulation of key eye-field transcription factors is controlled by the epigenetic modification of histone H3 acetylation through histone deacetylase activity. Our results identify an unexpected bioenergetic independent role of lactate as a signaling molecule necessary for mammalian eye morphogenesis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retina
/
Lactic Acid
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Nat Commun
Year:
2023
Document type:
Article