Highly efficient expression and secretion of human lysozyme using multiple strategies in Pichia pastoris.
Biotechnol J
; 18(11): e2300259, 2023 Nov.
Article
in En
| MEDLINE
| ID: mdl-37470505
ABSTRACT
BACKGROUND:
Human lysozyme (hLYZ), an emerging antibacterial agent, has extensive application in the food and pharmaceutical industries. However, the source of hLYZ is particularly limited.RESULTS:
To achieve highly efficient expression and secretion of hLYZ in Pichia pastoris, multiple strategies including G418 sulfate screening, signal sequence optimization, vacuolar sorting receptor VPS10 disruption, and chaperones/transcription factors co-expression were applied. The maximal enzyme activity of extracellular hLYZ in a shaking flask was 81,600 ± 5230 U mL-1 , which was about five times of original strain. To further reduce the cost, the optimal medium RDMY was developed and the highest hLYZ activity reached 352,000 ± 16,696.5 U mL-1 in a 5 L fermenter.CONCLUSION:
This research provides a very useful and cost-effective approach for the hLYZ production in P. pastoris and can also be applied to the production of other recombinant proteins.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Muramidase
/
Saccharomycetales
Limits:
Humans
Language:
En
Journal:
Biotechnol J
Year:
2023
Document type:
Article