CD95L concatemers highlight different stoichiometries of CD95-mediated apoptotic and nonapoptotic pathways.
Eur J Immunol
; 54(1): e2350626, 2024 Jan.
Article
in En
| MEDLINE
| ID: mdl-37837385
ABSTRACT
To better understand the stoichiometry of CD95L required to trigger apoptotic and nonapoptotic signals, we generated several CD95L concatemers from dimer to hexamer conjugated via a flexible link (GGGGS)2 . These ligands reveal that although the hexameric structure is the best stoichiometry to trigger cell death, a dimer is sufficient to induce the apoptotic response in CD95-sensitive Jurkat cells. Interestingly, only trimeric and hexameric forms can implement a potent Ca2+ response, suggesting that while CD95 aggregation controls the implementation of the apoptotic signal, both aggregation and conformation are required to implement the Ca2+ pathway.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apoptosis
/
Fas Receptor
Limits:
Humans
Language:
En
Journal:
Eur J Immunol
Year:
2024
Document type:
Article