Categorization of collagen type I and II blend hydrogel using multipolarization SHG imaging with ResNet regression.
Sci Rep
; 13(1): 19534, 2023 11 09.
Article
in En
| MEDLINE
| ID: mdl-37945626
ABSTRACT
Previously, the discrimination of collagen types I and II was successfully achieved using peptide pitch angle and anisotropic parameter methods. However, these methods require fitting polarization second harmonic generation (SHG) pixel-wise information into generic mathematical models, revealing inconsistencies in categorizing collagen type I and II blend hydrogels. In this study, a ResNet approach based on multipolarization SHG imaging is proposed for the categorization and regression of collagen type I and II blend hydrogels at 0%, 25%, 50%, 75%, and 100% type II, without the need for prior time-consuming model fitting. A ResNet model, pretrained on 18 progressive polarization SHG images at 10° intervals for each percentage, categorizes the five blended collagen hydrogels with a mean absolute error (MAE) of 0.021, while the model pretrained on nonpolarization images exhibited 0.083 MAE. Moreover, the pretrained models can also generally regress the blend hydrogels at 20%, 40%, 60%, and 80% type II. In conclusion, the multipolarization SHG image-based ResNet analysis demonstrates the potential for an automated approach using deep learning to extract valuable information from the collagen matrix.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hydrogels
/
Collagen Type I
Language:
En
Journal:
Sci Rep
Year:
2023
Document type:
Article