Rational design of humanized antibody inhibitors for cathepsin S.
Arch Biochem Biophys
; 751: 109849, 2024 01.
Article
in En
| MEDLINE
| ID: mdl-38061628
ABSTRACT
Cathepsin S (CTSS) is involved in pathogenesis of many human diseases. Inhibitors blocking its protease activity hold therapeutic potential. In comparison to small-molecule inhibitors, monoclonal antibodies capable of inhibiting CTSS enzymatic activity may possess advantageous pharmacological properties. Here we designed and produced inhibitory antibodies targeting human CTSS by genetically fusing the propeptide of procathepsin S (proCTSS) with antibodies in clinic. The resulting antibody fusions in full-length or fragment antigen-binding format could be stably expressed and potently inhibit CTSS proteolytic activity in high specificity. These fusion antibodies not only demonstrate a new approach for facile synthesis of antibody inhibitors against CTSS, but also represent novel anti-CTSS therapeutic candidates.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cathepsins
/
Antibodies, Monoclonal, Humanized
Limits:
Humans
Language:
En
Journal:
Arch Biochem Biophys
Year:
2024
Document type:
Article