Single Site N-Glycosylation of B Cell Maturation Antigen (BCMA) Inhibits γ-Secretase-Mediated Shedding and Improves Surface Retention and Cell Survival.
ACS Chem Biol
; 19(1): 153-161, 2024 Jan 19.
Article
in En
| MEDLINE
| ID: mdl-38085681
ABSTRACT
B cell maturation antigen (BCMA), a member of the tumor necrosis factor receptor (TNFR) family, on the cell surface plays a key role in maintaining the survival of plasma cells and malignant as well as inflammatory accessory cells. Therefore, targeting BCMA or disrupting its interaction with ligands has been a potential approach to cancer therapy. BCMA contains a single N-glycosylation site, but the function of N-glycan on BCMA is not understood. Here, we found that the N-glycosylation of BCMA promoted its cell-surface retention while removing the N-glycan increased BCMA secretion through γ-secretase-mediated shedding. Addition of γ-secretase inhibitor prevented nonglycosylated BCMA from shedding and protected cells from dexamethasone and TRAIL-induced apoptosis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
B-Cell Maturation Antigen
/
Multiple Myeloma
Limits:
Humans
Language:
En
Journal:
ACS Chem Biol
Year:
2024
Document type:
Article